Project

Part:BBa_K787003:Design

Designed by: Anish Vaghela   Group: iGEM12_Rutgers   (2012-10-02)


Bu-2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3303
    Illegal EcoRI site found at 6176
    Illegal EcoRI site found at 7384
    Illegal XbaI site found at 1156
    Illegal PstI site found at 1168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3303
    Illegal EcoRI site found at 6176
    Illegal EcoRI site found at 7384
    Illegal NheI site found at 3885
    Illegal NheI site found at 6170
    Illegal PstI site found at 1168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3303
    Illegal EcoRI site found at 6176
    Illegal EcoRI site found at 7384
    Illegal BamHI site found at 1150
    Illegal BamHI site found at 6109
    Illegal XhoI site found at 8252
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3303
    Illegal EcoRI site found at 6176
    Illegal EcoRI site found at 7384
    Illegal XbaI site found at 1156
    Illegal PstI site found at 1168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3303
    Illegal EcoRI site found at 6176
    Illegal EcoRI site found at 7384
    Illegal XbaI site found at 1156
    Illegal PstI site found at 1168
    Illegal NgoMIV site found at 1977
    Illegal NgoMIV site found at 4818
    Illegal NgoMIV site found at 6426
    Illegal NgoMIV site found at 6438
    Illegal NgoMIV site found at 8198
    Illegal AgeI site found at 2699
    Illegal AgeI site found at 3971
    Illegal AgeI site found at 4295
    Illegal AgeI site found at 5944
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1352
    Illegal BsaI site found at 1665
    Illegal BsaI site found at 8424
    Illegal SapI site found at 5926


Design Notes

Many illegal sites.


Source

Source: "Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways"

http://www.ncbi.nlm.nih.gov/pubmed/21358636

References